Feasibility Of Using Orange Juice For Digestion Of Swamp Eel Viscera For Harvesting Infective Stage Larva Of Gnathostoma Spp

نویسندگان

  • P Kue-A-Pai
  • V Wiwanitkit
چکیده

Gnathostoma spinigerum third stage larvae antigen is necessary for Western blot analysis in the diagnosis of Gnathostomiasis. Acid pepsin solution is required for digestion of eel's liver( Fluta alba ) to yield the larvae for antigen preparation. Here, he reported the feasibility of using orange juice as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third stage larvae. From the experimental digestion, it was found that we could recover larvae from orange juice. Thus, orange juice might be another choice for recovering Gnathostoma spinigerum third stage larvae.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Feasibility of using papaya skin extract for digestion of swamp eel viscera for harvesting infective stage larva of Gnathostoma spp.

In this study, we reported the feasibility of using papaya skin extract (Carica papaya L.) as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. From experimental digestion, we found that the different numbers of recovered larvae between papaya skin extract and pepsin were not statistically significant (p >0.05). When the derived larvae from pepsin and papa...

متن کامل

Contamination Of The Gnathostoma Spp Infective Stage Larvae In Fluta Alba Sold In Bangkok

In Thailand Gnathostoma spinigerum is the major causative agent of human gnathostomiasis. Usually, humans acquire the infection by consumption of G. spinigerum L3s infected hosts as partially cooked or uncooked food. Of several uncooked food, the highest prevalence of G. spinigerum L3s was found in Fluta alba (swamp eels). In Thailand, data of the prevalence and concentration of the parasite in...

متن کامل

Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar

The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval...

متن کامل

A survey of infective larvae of Gnathostoma in eels sold in Ho Chi Minh City.

To investigate the distribution of Gnathostoma spp in Ho Chi Minh City (HCM city), 1,081 eels were purchased from a local market twice a month from March 1998 to February 1999. Infective larvae of Gnathostoma spp detected from the flesh and liver of eels by the press preparation technique were examined and identified. Three hundred and fifty advanced third-stage larvae were recovered from liver...

متن کامل

بررسی لارو توکسوکارا در احشاء کبک های شکار شده

Abstract Background: Visceral larvae migrans (VLM) is the causative agent of acute and sub acute parasitic disease caused by the migration and establishment of second-stage larva of some Toxocara canis and Toxocara cati in human tissues. Human beings become infected by ingesting infective eggs or eating raw liver and heart of paratenic hosts (such as chicken, lamp and cow, etc) containing in...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2018